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Abstract: Objective ginsenoside Rh2 and cisplatin (Cisplatin, DDP) combination on laryngeal carcinoma cells Hep-2's role. Methods Cultured Hep-2 laryngeal carcinoma cells were added to a final concentration of 0.5,1.0,2.0,5.0 Î¼g / ml of DDP, a final concentration of 10,20,30,40 Î¼g / ml of Rh2, and the final concentration of 20Î¼g/ml of the joint final concentration of Rh2 were 0.5,1.0,2.0,5.0 Î¼g / ml of DDP, blank control group until 48 hours after drug, respectively, with an optical microscope , MTT, flow cytometry (Flow cytometry, FCM), fluorescence microscopy of cultured cells effect. The results can be seen under the light microscope the growth of normal laryngeal cell density, cells were spindle or polygonal cells in contact with each other. Rh2 in the low dose group and DDP group, showing a slight decrease of cell number, cell morphology did not change significantly. Rh2 in the high dose group and DDP group, cell number was significantly reduced, showing cell debris. While the low dose and low dose DDP Rh2 in combination, the availability and the high dose group and DDP group Rh2 same effect. MTF results showed that Rh2, DDP alone and in combination were tested dose dependent increase in cell growth inhibition of laryngeal cancer. Rh2 low dose and low dose DDP in combination, can inhibit the growth of laryngeal cancer cells. FCM showed Rh2 and DDP alone, can induce apoptosis, while the combined availability of more obvious than that alone induce apoptosis effect. Acridine orange staining showed that the treatment group were seen budding cell membrane, chromatin condensation, nuclear fragmentation and apoptotic bodies and other typical features of apoptosis. Conclusion Ginsenoside Rh2 and DDP can induce apoptosis in laryngeal cancer cells, the two combined to reveal a synergistic effect, can play a more significant role in cancer.
Central South University Admissions Information Admissions Department Name Major Name Year research notes to be subject of examination in 2009 enrollment Pathology and Pathophysiology, Central South University, 01 digestive tumor pathology (Department of Pathology, Department of Pathology, Xiangya Hospital) 02 lung tumor pathology (disease Faculty of Science, Department of Pathology, Xiangya Hospital) 03 silicosis Science (Department of Pathology, Department of Pathology, Xiangya Hospital) 04 Clinical Pathology (Department of Pathology, Department of Pathology, Xiangya Hospital) 05 Ultrastructural Pathology (Medical Ultrastructure Ultrastructural Pathology Lab) 06 septic shock (Pathophysiology) 07 cardiovascular pathophysiology (Pathophysiology) 13 â‘ 101 political â‘¡ 201 Integrated Traditional and Western English â‘¢ 306 (657) Central South University Admissions Information Admissions Department Name Major Name Year research Notes to be
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