15:29,18,Dec,2009 | (2892/0/0) | Original
Note: The trackback url will expire after 23:59:59 today
Note: The trackback url will expire after 23:59:59 today
Abstract: Objective ginsenoside Rh2 and cisplatin (Cisplatin, DDP) combination on laryngeal carcinoma cells Hep-2's role. Methods Cultured Hep-2 laryngeal carcinoma cells were added to a final concentration of 0.5,1.0,2.0,5.0 Î¼g / ml of DDP, a final concentration of 10,20,30,40 Î¼g / ml of Rh2, and the final concentration of 20Î¼g/ml of the joint final concentration of Rh2 were 0.5,1.0,2.0,5.0 Î¼g / ml of DDP, blank control group until 48 hours after drug, respectively, with an optical microscope , MTT, flow cytometry (Flow cytometry, FCM), fluorescence microscopy of cultured cells effect. The results can be seen under the light microscope the growth of normal laryngeal cell density, cells were spindle or polygonal cells in contact with each other. Rh2 in the low dose group and DDP group, showing a slight decrease of cell number, cell morphology did not change significantly. Rh2 in the high dose group and DDP group, cell number was significantly reduced, showing cell debris. While the low dose and low dose DDP Rh2 in combination, the availability and the high dose group and DDP group Rh2 same effect. MTF results showed that Rh2, DDP alone and in combination were tested dose dependent increase in cell growth inhibition of laryngeal cancer. Rh2 low dose and low dose DDP in combination, can inhibit the growth of laryngeal cancer cells. FCM showed Rh2 and DDP alone, can induce apoptosis, while the combined availability of more obvious than that alone induce apoptosis effect. Acridine orange staining showed that the treatment group were seen budding cell membrane, chromatin condensation, nuclear fragmentation and apoptotic bodies and other typical features of apoptosis. Conclusion Ginsenoside Rh2 and DDP can induce apoptosis in laryngeal cancer cells, the two combined to reveal a synergistic effect, can play a more significant role in cancer.
Central South University Admissions Information Admissions Department Name Major Name Year research notes to be subject of examination in 2009 enrollment Pathology and Pathophysiology, Central South University, 01 digestive tumor pathology (Department of Pathology, Department of Pathology, Xiangya Hospital) 02 lung tumor pathology (disease Faculty of Science, Department of Pathology, Xiangya Hospital) 03 silicosis Science (Department of Pathology, Department of Pathology, Xiangya Hospital) 04 Clinical Pathology (Department of Pathology, Department of Pathology, Xiangya Hospital) 05 Ultrastructural Pathology (Medical Ultrastructure Ultrastructural Pathology Lab) 06 septic shock (Pathophysiology) 07 cardiovascular pathophysiology (Pathophysiology) 13 â‘ 101 political â‘¡ 201 Integrated Traditional and Western English â‘¢ 306 (657) Central South University Admissions Information Admissions Department Name Major Name Year research Notes to be
ã€Similar documentsã€‘ China Academic Journal ago 10 1 ZHU Pei, Li Ao, Jiang Kun Yuan, training Wei Kun, Chen Faming, WU Zhen, Wang Tianyi, You Zhongyi, Li Jin, the Xiao Limin; severe respiratory steam burn animals (dogs) model the production of [J ]; Third Military Medical University; 1980 04 Chapter 2 weeks of good, Ai Ling billion, Peng Gaosheng; severe steam burns of respiratory Six experimental study - the advantage of early respiratory pathogen in dogs burn [J]; Third Military Medical University; 1980 04 3 Dongyan Lin, Tang success; severe steam burns of respiratory eight experimental study - early red blood cell 2,3 - diphosphoglycerate acid content [J]; Third Military Medical University; 1980 04 4 Jiang Kun-yuan, Li Ao, ZHU Pei, training Wei Kun, Li Jin, the Xiao Limin, Wu Zhenzhong, Chen Faming, You Zhongyi, Wang Tianyi, Li Shaoqin, Li Wenqing, Yang Dong, Zhou Zonghai, Liming You; severe respiratory steam burns experimental study of eleven - the blood biochemical and osmotic pressure analysis [J]; Third Military Medical University; 1980 04 5 Shen Wei Ning; respiratory tract infection with Staphylococcus aureus tracheotomy (with report of two cases) [J]; Bengbu Medical College; 1980 03 6 Mo Xiu-Fen; respiratory and immune [J]; Jilin University (Medical Sciences); 1980 04 7 Chen Anliang; CJFD1980 [J]; Guangdong Medicine; 1980 03 8; tracheal instillation of methylcholanthrene induced by gold Hamster preliminary study of experimental lung cancer [J]; Kunming Medical College; 1980 01 9 children and;; guinea pig respiratory tract exposure to sulfuric acid mist when the damage [J]; Railway Occupational Safety Health and Environmental Protection; 1980 02 more than 10 field pool; Fu Weizu;; organic solvent percutaneous absorption [J]; foreign medicine. Health Toxicology; 1980, 01 of China's important conference papers Full-text database ago 10 1 Xusan Lin; WU RenLiang; Chen Chunlian; Hao Chunrong;; cadherin factor in the smoking mice with airway epithelial cell injury and repair of expression [A]; Chinese Journal of Physiology Vocational Education Committee at its seventh Symposium Papers Series [C]; 1999 2 Ding Hing; T. T. Union;; of laryngeal cancer after the nutritional [A]; Chinese Nutrition Society of the Seventh National Conference on Nutrition Abstracts of the [C]; 1996 3 Yue Yi;; respiratory chemical burns [A]; national third burn Surgical academic conference abstracts [C]; 1991 4 Luo groups; Zhou Pengxiang; Quan-Yong;; oliguria for 53 days and five organ failure in severe burn patients rescued [A]; Chinese Medical Association of Academic Surgery, the Fifth National Burn Conference Papers Series [C]; 1997 years 5 Xia Xiaolian;; inhalation injury care to prevent respiratory tract hemorrhage [A]; Chinese Medical Association Fifth National Academic Conference Papers assembly burn surgery [C]; 1997 6 Li Yanzhen; Shu-lan; ; successful nursing four cases of large area contact with gasoline experience skin damage [A]; Chinese Medical Association Fifth National Academic Conference Papers assembly burn surgery [C]; 1997 7 Zhangxiu Jun;; inhalation injury tracheotomy care [A ]; the Chinese Medical Association Fifth National Academic Conference Papers assembly burn surgery [C]; 1997 8 Xing Lisheng; Niezhong Qi;; lung clinical analysis of 45 cases of encephalopathy [A]; Chinese Medical Association of the Fifth National Conference on Emergency Medicine Proceedings [C]; 1994 9 Liu Ping; Huang Jianyun; Bao-Xue Ming;; acute toxicity of p-nitroaniline (report of 6 cases) [A]; Chinese Medical Association of the Fifth National Conference on Emergency Medicine Proceedings [C ]; 1994 Chapter 10 Kui Lun; Xu Wan Zhen;; acute dimethyl formamide poisoning treatment [A]; Chinese Medical Association of the Fifth National Emergency Medicine Conference Proceedings [C]; 1994 China's major newspapers, full-text database, the first 10 1 Rituximab; gas poisoning after the . [N]; health Times; to Weiyou Kui Zhang 2, 2004; porcine respiratory syndrome in the differential diagnosis [N]; China Animal Husbandry reported; 3 Sheung Yee Yong 2004; cough busy Mo cough [N]; public health reporting; 4, 2004, Shanxi Tumor Hospital, Professor MI Zhen States; in innovation and brand development to enhance the hospital [N]; Guangming Daily; 5, 2004, Xiao Wei; autumn to strengthen the nose Health Care [N]; Hebei Science and Technology News; 2004 6 HE Quan-ying Tan Xingyu; COPD patients using immunomodulators [N]; Health News; 7, 2004 by reporter leaves; pet will get a cold? [N]; Healthy Times; 2004 Jiangsu Academy of Agricultural Sciences 8, all veterinary Yumei; Bordetella rabbit bronchial disease [N]; of Agricultural Science and Technology News; 2004 9 Zhimin; control of bovine respiratory disease the birth of new vaccines [N]; Farmer Daily; 2004 10, medical director of Chinese Medicine Hospital, Zhabei District, Shanghai, associate professor, deputy director of Physicians Cai Jinwei; Chinese Qiaozhi intractable hiccups [N]; Shanghai Traditional Chinese Medicine reported; full paper in China in 2004 PhD Database ago 10 1 Guo Rui; treatment of recurrent laryngeal analysis of [D]; PLA Postgraduate Medical School; 2003 2 Zhou Hui; rat Î²-defensin-2 gene expression and regulation in the respiratory tract and transgenic of [D]; Sichuan University; 2003 3 Zhang; airway inflammation etiology mechanism [D]; Xinjiang Medical University; 2005 4 Yellow; community-acquired pneumonia etiology of [D]; Second Military Medical University; 5, 2005, Ding Jianzhong; outside the pathogenesis of experimental study dry [D]; College of Traditional Chinese; 6 Dongjin Tang 2006; the carcinogenic effects of arsenic and mechanism of [D]; Peking Union Medical College; 1989 7, GUO tidal wave; human mast cells and basophils clinical, cellular and molecular level [D]; Peking Union Medical College; 8, 1994, Yang Xiaoyan; domestic ducks infected virulent duck plague the dynamic distribution of the intestinal mucosal immunity and Road flora structure [D]; Sichuan Agricultural University; 9, 2006 Zhang Lei; Yu Ping Feng San immune mechanism of action [D]; Chengdu University of TCM; 2006 10 Chen Zhihong; AQP5 in human airway submucosal gland cells in the water cross- membrane permeability and mucin synthesis, secretion of [D]; Fudan University; degree in China in 2006 before the database full paper 10 1 leaf green; perfluorocarbon partial liquid ventilation on the respiratory inhalation injury in dogs Histopathology of [D]; Jiangxi Medical College; 2, 2001, Chen Wenqiang; tumor associated with febrile respiratory tract infection syndromes of [D]; Beijing University of Chinese Medicine; 2002 3 Huangjing Bin; Jinshuiliujunjian respiratory tract in mice with chronic bronchitis Influence of cilia [